The inhibitory effect of ginsan on TGF-β mediated fibrotic process

• Published in: Journal of cellular physiology 2011-05, Vol.226 (5), p.1241-1247
• Main Authors: Ahn, Ji-Yeon, Kim, Mi-Hyoung, Lim, Min-Jin, Park, Sarah, Lee, Sae-lo-oom, Yun, Yeon-Sook, Song, Jie-Young
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Animals; Bleomycin; Collagen Type I - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Extracellular Signal-Regulated MAP Kinases - metabolism; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Fibronectins - metabolism; Fibrosis; Genes, Reporter; Humans; Lung - drug effects; Lung - metabolism; Lung - pathology; Male; Mice; Mice, Inbred C57BL; NIH 3T3 Cells; Phosphorylation; Polysaccharides - pharmacology; Protein-Serine-Threonine Kinases - drug effects; Protein-Serine-Threonine Kinases - metabolism; Proteoglycans - drug effects; Proteoglycans - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Pulmonary Fibrosis - chemically induced; Pulmonary Fibrosis - metabolism; Pulmonary Fibrosis - pathology; Pulmonary Fibrosis - prevention & control; Receptors, Transforming Growth Factor beta - drug effects; Receptors, Transforming Growth Factor beta - metabolism; Recombinant Proteins - metabolism; Signal Transduction - drug effects; Smad2 Protein - genetics; Smad2 Protein - metabolism; Smad3 Protein - genetics; Smad3 Protein - metabolism; Time Factors; Transfection; Transforming Growth Factor beta1 - metabolism
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.22452

Transforming growth factor‐beta (TGF‐β) plays a central role in the development of fibrosis by stimulating extracellular matrix accumulation, and signals either directly or indirectly through types I, II, and III (TβRI, II, and III) TGF‐β receptor complexes. Ginsan, a polysaccharide extracted from Panax ginseng, has multiple immunomodulatory effects. Here, we examine whether ginsan regulates the fibrogenic process by interfering with TGF‐β signaling pathways. TGF‐β treatment of murine or human normal lung fibroblasts enhanced the levels of several fibrotic markers, including smooth muscle alpha actin (α‐SMA), collagen‐1, and fibronectin. Interestingly, ginsan treatment either before or after TGF‐β administration led to significant reductions in all of α‐SMA, collagen‐1, and fibronectin expression levels. Ginsan not only inhibited phosphorylation of Smad2 and Smad3, but also attenuated pERK and pAKT signaling induced by TGF‐β. Moreover, ginsan restored TβRIII protein expression, which was significantly downregulated by TGF‐β, but reduced TβRI and TβRII protein levels. In a murine model of bleomycin (BLM)‐induced pulmonary fibrosis, ginsan significantly suppressed accumulation of collagen, α‐SMA, and TGF‐β. These data collectively suggest that ginsan acts as an effective anti‐fibrotic agent in the treatment of pulmonary fibrosis by blocking multiple TGF‐β signaling pathways. J. Cell. Physiol. 226: 1241–1247, 2011. © 2010 Wiley‐Liss, Inc.