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Effect of addition of Ca 2+ to titanium by a hydrothermal method on soft tissue sealing

The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca ) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectro...

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Bibliographic Details
Published in:Microscopy research and technique 2022-09, Vol.85 (9), p.3050-3055
Main Authors: Fu, Zhendi, Deng, Xuehua, Fang, Xiaodan
Format: Article
Language:English
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Summary:The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca ) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy; proliferation of hGF-1 cells was measured by the CCK-8 assay; immunofluorescence staining was done to detect adherent proteins on titanium surface. In vivo, the degree of attachment between the implant and the surrounding soft tissue was measured by horseradish peroxidase (HRP). The percentage of hGF-1 cells adhering in the Ca group was significantly higher (p 
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.24152