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Effect of addition of Ca 2+ to titanium by a hydrothermal method on soft tissue sealing
The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca ) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectro...
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Published in: | Microscopy research and technique 2022-09, Vol.85 (9), p.3050-3055 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca
) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy; proliferation of hGF-1 cells was measured by the CCK-8 assay; immunofluorescence staining was done to detect adherent proteins on titanium surface. In vivo, the degree of attachment between the implant and the surrounding soft tissue was measured by horseradish peroxidase (HRP). The percentage of hGF-1 cells adhering in the Ca group was significantly higher (p  |
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ISSN: | 1059-910X 1097-0029 |
DOI: | 10.1002/jemt.24152 |