Simple single-step single-enzyme synthesis of [14C]-GSH

The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant...

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Bibliographic Details
Published in:Journal of labelled compounds & radiopharmaceuticals 2010-03, Vol.53 (3), p.110-112
Main Authors: de Keczer, Steve A., Voronin, Tatyana, Yao, Jennifer, Zhang, Fang-Jie, Masjedizadeh, Mohammad R.
Format: Article
Language:English
Subjects:
[14C]-glutathione
[14C]-glycine
[14C]-GSH
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Contrast media. Radiopharmaceuticals
Fundamental and applied biological sciences. Psychology
GSH synthetase
L-γ-glutamyl-L-cysteine
Medical sciences
Methods. Procedures. Technologies
Pharmacology. Drug treatments
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Description
Summary:The tri‐peptide [14C]‐glutathione ([14C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L‐γ‐glutamyl‐L‐cysteine and [14C]‐glycine. Preparative reverse phase HPLC afforded [14C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [14C]‐GSH to the disulfide [14C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd. An efficient one‐step method for the cell‐free preparation of [14C]‐GSH from readily available reagents: [14C]‐gly and γ‐glu‐cys and catalyzed by glutathione synthetase in a simplified cocktail is described. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.1735