Increased selectivity in inflammatory site identification via labelling of IgG with N-succinimidyl-4-[125I]iodobenzoate

Human nonspecific polyclonal IgG was labelled with 125I through direct and indirect labelling methods using chloramine‐T and a nonphenolic radioiodinated intermediate N‐succinimidyl‐4‐[125I]iodobenzoate (125I‐SIB), respectively. Tissue distribution of radioiodinated IgG was assessed in normal and in...

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Bibliographic Details
Published in:Journal of labelled compounds & radiopharmaceuticals 2002-10, Vol.45 (11), p.927-934
Main Authors: Beiki, Davood, Shahhosseini, Soraya, Khalaj, Ali, Eftekhari, Mohammad
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Glycoproteins
human nonspecific polyclonal IgG
in vivo deiodination
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
N-succinimidyl-4-iodobenzoate
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Proteins
turpentine
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Summary:Human nonspecific polyclonal IgG was labelled with 125I through direct and indirect labelling methods using chloramine‐T and a nonphenolic radioiodinated intermediate N‐succinimidyl‐4‐[125I]iodobenzoate (125I‐SIB), respectively. Tissue distribution of radioiodinated IgG was assessed in normal and induced inflammation mice. Although, radioiodinated IgG accumulated in the inflammatory area, results showed decreased thyroid and stomach activity and improved inflammatory thigh‐to‐normal tissue ratios with the indirect labelling method (125I‐IB‐IgG) compared with the direct labelling method (125I‐IgG), indicating reduced in vivo deiodination. These results indicate that the 125I‐SIB is probably a preferable approach for labelling antibodies with iodine radioisotopes. Copyright © 2002 John Wiley & Sons, Ltd.
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.608