Molecular analysis of transmission of hepatitis C virus in a nurse who acquired acute hepatitis C after caring for a viremic patient with epistaxis

• Published in: Journal of medical virology 2009-08, Vol.81 (8), p.1363-1370
• Main Authors: Toda, Takayuki, Mitsui, Takehiro, Tsukamoto, Yukie, Ebara, Takeshi, Hirose, Akinori, Masuko, Kazuo, Nagashima, Shigeo, Takahashi, Masaharu, Okamoto, Hiroaki
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Amino Acid Sequence; Biological and medical sciences; Cluster Analysis; Epidemiology; Epistaxis; Female; finger injury; Fundamental and applied biological sciences. Psychology; genotype; Hepacivirus - classification; Hepacivirus - isolation & purification; Hepatitis C - transmission; Hepatitis C - virology; hepatitis C virus; Human viral diseases; Humans; Infectious diseases; Male; Medical sciences; Microbiology; Middle Aged; Molecular Epidemiology; Molecular Sequence Data; Nurses; Occupational Exposure; patient- to-nurse transmission; phylogenetic analysis; Phylogeny; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Viral diseases; Viral hepatitis; Viral Nonstructural Proteins - genetics; Virology; Young Adult
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.21537

A 23-year-old nurse (HC-IP) developed acute hepatitis C. Intrafamilial transmission of hepatitis C virus (HCV) was suspected initially because her parents were carriers of HCV of the same genotype (1b) as that of Patient HC-IP. However, the HCV isolate from Patient HC-IP and those from her parents shared identities of only 92.4-92.7% in the 1,087-nucleotide (nt) sequence within the NS5B region. It was then suspected that she contracted HCV infection during medical practice. Sixteen patients with antibodies to HCV (anti-HCV) were hospitalized 1-3 months before she became positive for anti-HCV. Upon analysis of stored serum samples, 14 of the 16 patients were found to be positive for HCV RNA, and 9 of the 14 viremic patients had genotype 1b HCV. Although the shared identities between the HCV isolate from Patient HC-IP and those from eight of the nine patients were merely 90.6-93.9% within the 1,087-nt NS5B sequence, the HCV isolate from the remaining one patient (HC-P12) was 99.7% identical to that from Patient HC-IP. Upon analysis of the E1 and E2 junctional region including hypervariable region 1 (283 nt), there was a close relationship (99.3-100%) between clones obtained from Patients HC-IP and HC-P12. Although the nurse HC-IP had a finger injury, she took care of Patient HC-P12, a 70-year-old man with HCV-related cirrhosis and recurrent epistaxis, occasionally without wearing protective gloves. This study indicates the occurrence of HCV transmission by exposure of nonintact skin to blood in health care settings. J. Med. Virol. 81:1363-1370, 2009.