Role of mutations identified in ORFs M27, M36, m139, m141, and m143 in the temperature‐sensitive phenotype of murine cytomegalovirus mutant tsm 5

• Published in: Journal of medical virology 2012-06, Vol.84 (6), p.912-922
• Main Authors: Al‐Ali, Abdulaziz, Timoshenko, Olga, Martin, Brian A.B., Sweet, Clive
• Format: Article
• Language: English
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.23273

A mutant of murine cytomegalovirus (MCMV), tsm 5, which is temperature‐sensitive for replication in murine embryo fibroblasts at 40°C, failed to replicate to detectable levels in mice. A total of 18 non‐synonymous mutations have been identified in tsm 5. In a previous study, a mutation (C890Y) identified in the M70 primase gene, when introduced into the wt M70 primase, resulted in a mutant with reduced viral replication at 40°C in vitro and which was severely attenuated in vivo. Five other previously identified mutations may also contribute to the tsm 5 phenotype: (1) an A658S mutation in a protein expressed by the M27 ORF; (2) a V54I mutation in M36; (3) a Y565* mutation in m139; (4) a V195M mutation in m141; and (5) an M232I mutation in m143. In the present study, the above‐mentioned mutations were introduced individually (M27, M36, m139, m141, m143) or together (M27/M36) into the MCMV K181 (Perth) variant bacterial artificial chromosome (BAC) using RecE/T homologous recombination. Growth in culture revealed that, apart from the double mutant (M27 and M36) and the m139 mutant, the introduced mutations in the above‐mentioned genes did not show a temperature‐sensitive phenotype in MEF or Raw 264.7 macrophage cells compared to their revertants or the wt virus. In contrast, replication of the M27/M36 double mutant was drastically reduced in MEFs at 40°C and in macrophages at 37°C. Replication of the m139 mutant was reduced in MEF cells at 40°C but not in macrophages. Thus, at least three further mutations contribute to the tsm 5 phenotype. J. Med. Virol. 84:912–922, 2012. © 2012 Wiley Periodicals, Inc.