Biophysical Characterization of PEI/DNA Complexes

The main goal of this study was to determine the effects of polyethylenimine (PEI) molecular weight and structure (750kDa, 25kDa, 2kDa branched, and 25kDa linear PEI) and the nitrogen/phosphate (N/P) molar ratio on the physical properties and transfection efficiencies of PEI/DNA complexes. Fourier t...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2003-08, Vol.92 (8), p.1710-1722
Main Authors: Choosakoonkriang, Sirirat, Lobo, Brian A., Koe, Gary S., Koe, Janet G., Middaugh, C.Russell
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biophysical Phenomena
Biophysics
Cercopithecus aethiops
CHO Cells
circular dichroism
COS Cells
Cricetinae
differential scanning calorimetry
DNA - analysis
DNA - chemistry
DNA - genetics
DNA delivery
FTIR
gene delivery
General pharmacology
Genetic Therapy - methods
isothermal titration calorimetry
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
physical characterization
plasmid DNA
Polyethyleneimine - analysis
Polyethyleneimine - chemistry
polyethylenimine
Spectroscopy, Fourier Transform Infrared - methods
Transfection - methods
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Summary:The main goal of this study was to determine the effects of polyethylenimine (PEI) molecular weight and structure (750kDa, 25kDa, 2kDa branched, and 25kDa linear PEI) and the nitrogen/phosphate (N/P) molar ratio on the physical properties and transfection efficiencies of PEI/DNA complexes. Fourier transform infrared spectroscopy revealed that DNA remained in the B conformation when complexed to all PEIs. Unique alterations in the circular dichroism spectra of DNA were observed in the presence of each PEI, whereas differential scanning calorimetry measurements showed that all PEIs examined destabilized supercoiled DNA at N/P
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.10437