Chromatographic Analysis of Phenobarbital and Belladonna Alkaloid Combinations

A partition column chromatographic technique is applied to the analysis of phenobarbital and the belladonna alkaloids as found in commercial tablets and elixirs. Separation is achieved on a Celite column in which the stationary phase is a 10 per cent solution of p-toluenesulfonic acid. Ether and chl...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1965-07, Vol.54 (7), p.1046-1049
Main Authors: Koch, Stanley A., Levine, Joseph, Zenker, Nicholas
Format: Article
Language:English
Subjects:
Atropa belladonna
Chromatography
Phenobarbital
Plants, Medicinal
Plants, Toxic
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Summary:A partition column chromatographic technique is applied to the analysis of phenobarbital and the belladonna alkaloids as found in commercial tablets and elixirs. Separation is achieved on a Celite column in which the stationary phase is a 10 per cent solution of p-toluenesulfonic acid. Ether and chloroform elute phenobarbital and the alkaloid–p-toluenesulfonic acid complex, respectively. The isolated phenobarbital is determined spectrophotometrically, and the total belladonna alkaloids are measured colorimetrically by a modification of a published procedure. A study of the modifications is reported and statistically evaluated. Standard recoveries averaged 99.01 per cent for phenobarbital and 98.73 per cent for atropine sulfate.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600540722