High-Performance Liquid Chromatographic Analysis of Iodochlorohydroxyquin and Hydrocortisone in Ointments and Creams

• Published in: Journal of pharmaceutical sciences 1983-09, Vol.72 (9), p.1036-1039
• Main Authors: Ezzedeen, F.W., Stohs, S.J., Masoud, A.N.
• Format: Article
• Language: English
• Subjects: high-performance liquid chromatography; High-performance liquid chromatography-simultaneous determination of iodochlorhydroxyquin and hydrocortisone; High-performance liquid chromatography—simultaneous determination of iodochlorhydroxyquin and hydrocortisone, ointment and cream; Hydrocortisone-simultaneous determination with iodochlorhydroxyquin; Hydrocortisone—simultaneous determination with iodochlorhydroxyquin, ointment and cream, high-performance liquid chromatography; Iodochlorhydroxyquin-simultaneous determination with hydrocortisone; Iodochlorhydroxyquin—simultaneous determination with hydrocortisone, ointment and cream, high-performance liquid chromatography; ointment and cream
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.2600720918

□ A simple isocratic, high-performance liquid chromatographic (HPLC) assay procedure was developed for the simultaneous determination of iodochlorhydroxyquin and hydrocortisone in ointments and creams using phenyl salicylate as an internal standard. Ointment samples were extracted by direct dissolution in ether. Homogeneous suspensions of the creams were prepared in the mobile phase. The samples were spiked by the addition of standard iodochlorhydroxyquin, standard hydrocortisone, and the internal standard and subsequently extracted with the mobile phase. HPLC was performed using a reverse-phase microparticulate C-18 column, a precolumn, and a UV detector set at 256nm. A mobile phase containing methanol and 0.05M phosphoric acid (70:30) was employed at a flow rate of 1ml/min. The percent iodochlorhydroxyquin and hydrocortisone found to be present in eight commercial products is reported.