High-Performance Liquid Chromatographic Determination of Tracazolate and Its Major Metabolite in Plasma

A high-performance liquid chromatographic (HPLC) method is reported that provides for either separate or simultaneous plasma determination of tracazolate and its major metabolite, the free carboxylic acid. Tracazolate is extracted from plasma with hexane and is quantitated by reverse-phase HPLC usin...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences 1984-05, Vol.73 (5), p.667-670
Main Authors: Hermes, M., Malbica, J.O.
Format: Article
Language:English
Subjects:
Animals
Anti-Anxiety Agents
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Dogs
Drug Stability
General pharmacology
HPLC
HPLC-tracazolate and its free carboxylic acid metabolite
HPLC—tracazolate and its free carboxylic acid metabolite, individual and simultaneous assay in plasma
Hydrogen-Ion Concentration
individual and simultaneous assay in plasma
Kinetics
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Pyrazoles - blood
Spectrophotometry, Ultraviolet
Tracazolate-free carboxylic acid metabolite
Tracazolate—free carboxylic acid metabolite, individual and simultaneous assay in plasma, HPLC
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A high-performance liquid chromatographic (HPLC) method is reported that provides for either separate or simultaneous plasma determination of tracazolate and its major metabolite, the free carboxylic acid. Tracazolate is extracted from plasma with hexane and is quantitated by reverse-phase HPLC using an internal standard. The metabolite is extracted in an additional step, methylated, and either quantitated using the same HPLC conditions in a separate determination or combined with the hexane extract for the simultaneous HPLC determination of drug and metabolite. The plasma concentrations of metabolite were as much as 60 times greater than free drug in some animal species, therefore requiring the use of separate determinations for the two compounds. The analyses have limits of reproducible quantitation of 20 ng/mL for tracazolate and 50 ng/mL for the metabolite in plasma. The simultaneous analysis for tracazolate and the metabolite was used to evaluate plasma levels of both compounds versus time and dose. Data generated in conjunction with a toxicology study are reported to demonstrate the applicability of the procedure.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600730519