Overexpression of ZEB1 relates to metastasis and invasion in osteosarcoma

• Published in: Journal of surgical oncology 2012-06, Vol.105 (8), p.830-834
• Main Authors: Shen, Aidong, Zhang, Yunqing, Yang, Huiguang, Xu, Ruisheng, Huang, Guowei
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Blotting, Western; Bone and Bones - metabolism; Bone and Bones - pathology; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Bone Neoplasms - pathology; Cells, Cultured; Child; Disease Progression; Female; Follow-Up Studies; Gene Expression Regulation, Neoplastic; Homeodomain Proteins - antagonists & inhibitors; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - secondary; Male; Neoplasm Invasiveness; Neoplasm Staging; osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - secondary; Prognosis; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA interference; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Transcription Factors - antagonists & inhibitors; Transcription Factors - genetics; Transcription Factors - metabolism; Young Adult; ZEB1; Zinc Finger E-box-Binding Homeobox 1
• ISSN: 0022-4790; 1096-9098
• DOI: 10.1002/jso.23012

Background This study aimed to investigate the expression of ZEB1 in osteosarcoma tissues and to discuss the relationship between ZEB1 expression and osteosarcoma metastasis. Methods Using RT‐PCR and Western blotting, the mRNA and protein expressions of ZEB1 in the osteosarcoma and normal bone tissues were detected. Using the RNA interference technique, the expression of ZEB1 in the human osteosarcoma MG‐63 cell line was downregulated, and the changes in the invasion of MG‐63 cells were examined. Results The positive mRNA expression rate of ZEB1 in the osteosarcoma tissues was significantly higher than that in normal bone tissue (P