Analysis of alkaloids in Ipecacuanhae radix and preparations by capillary zone electrophoresis

A capillary zone electrophoresis method for quantitation of the alkaloids emetine and cephaeline in Ipecacuanhae radix fluid extract and Ipecac syrup using papaverine as internal standard is described. Baseline separation was achieved within 10 min using a fused silica capillary and a running electr...

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Bibliographic Details
Published in:Journal of separation science 2003-08, Vol.26 (12-13), p.1175-1179
Main Authors: Sturm, Sonja, Taglioli, Vania, Bilia, Anna R., Vincieri, Francesco, Stuppner, Hermann
Format: Article
Language:English
Subjects:
Biological and medical sciences
Capillary zone electrophoresis
Cephaeline
Emetine
General pharmacology
Ipecac syrup
Ipecacuanhae extractum fluidum
Ipecacuanhae Rad
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:A capillary zone electrophoresis method for quantitation of the alkaloids emetine and cephaeline in Ipecacuanhae radix fluid extract and Ipecac syrup using papaverine as internal standard is described. Baseline separation was achieved within 10 min using a fused silica capillary and a running electrolyte consisting of citric acid (50 mM), disodium hydrogenphosphate (100 mM), and 2.5% methanol (pH 4.4). Regression equations revealed a linear response from 0.05 to 1.9 mg/mL for cephaeline and emetine (correlation coefficients 0.9991–0.9993). The lowest quantitation limits were determined as 0.03 mg/mL for both alkaloids, and the limit of detection was 0.012 mg/mL. The day‐to‐day variations of retention times were  99.8%.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200301199