Determination of capsaicinoids in C apsicum species using ultra performance liquid chromatography‐mass spectrometry

In the present work, a rapid and sensitive ultra performance liquid chromatography‐mass spectrometry method has been proposed for the analysis of capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin) present in different C apsicum samples. Extracti...

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Bibliographic Details
Published in:Journal of separation science 2012-11, Vol.35 (21), p.2892-2896
Main Authors: Alothman, Zeid Abdullah, Wabaidur, Saikh Mohammad, Khan, Mohammad Rizwan, Ghafar, Ayman Abdel, Habila, Mohamed Abdelaty, Ahmed, Yacine Badjah Hadj
Format: Article
Language:English
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Summary:In the present work, a rapid and sensitive ultra performance liquid chromatography‐mass spectrometry method has been proposed for the analysis of capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin) present in different C apsicum samples. Extraction of capsaicinoids was carried out by liquid–liquid extraction using ethanol as an extracting solvent, while the chromatographic separation was achieved by reversed phase C 18 column with gradient mobile phase (solvent A: acetonitrile and solvent B: water with 0.1% formic acid). Under the optimum experimental conditions, the linear ranges were 0.5–50 μg/g with correlation coefficient ( r 2 ) >0.999 for each capsaicinoids and detection limits were 0.15, 0.05, 0.06, 0.2, and 0.1 μg/g for nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin, respectively. Run‐to‐run and day‐to‐day precisions of the method with relative standard deviations
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201200459