UV Spectrophotometry: Improvements in the Study of the Degree of Acetylation of Chitosan

A simple and rapid method for determining the degree of N‐acetylation (DA) of chitosan by ultraviolet spectrophotometry using double beam equipment is proposed. D‐Glucosamine (GlcN) hydrochloride and N‐acetylglucosamine (GlcNAc) were used as model substances, and chitosan solutions in different solv...

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Bibliographic Details
Published in:Macromolecular bioscience 2003-10, Vol.3 (10), p.531-534
Main Authors: Pedroni, Viviana Isabel, Gschaider, María Elena, Schulz, Pablo Carlos
Format: Article
Language:English
Subjects:
Applied sciences
biopolymers
chitosan
conductometric titration
degree of acetylation
Exact sciences and technology
Natural polymers
Physicochemistry of polymers
Starch and polysaccharides
UV-vis spectroscopy
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Summary:A simple and rapid method for determining the degree of N‐acetylation (DA) of chitosan by ultraviolet spectrophotometry using double beam equipment is proposed. D‐Glucosamine (GlcN) hydrochloride and N‐acetylglucosamine (GlcNAc) were used as model substances, and chitosan solutions in different solvents were studied. The best results were obtained from 0.1 M HCl solutions, with high reproducibility and in good agreement with those given by conductometric titration, FT‐IR, 1H NMR and elemental analysis. Correlation between DA obtained by UV and DA obtained by conductometric titration.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.200300024