Orientation‐Selective Incorporation of Transmembrane F 0 F 1 ATP Synthase Complex from Micrococcus luteus in Polymer‐Supported Membranes

We report the vectorial incorporation of a highly asymmetric F 0 F 1 ATP synthase complex from Micrococcus luteus into polymer‐supported membranes. Dynamic light scattering and cryo electron microscopy confirm that the use of weak surfactants (bile acid) allows for the non‐disruptive protein incorpo...

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Bibliographic Details
Published in:Macromolecular bioscience 2008-11, Vol.8 (11), p.1034-1043
Main Authors: Tutus, Murat, Rossetti, Fernanda F., Schneck, Emanuel, Fragneto, Giovanna, Förster, Friedrich, Richter, Ralf, Nawroth, Thomas, Tanaka, Motomu
Format: Article
Language:English
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Summary:We report the vectorial incorporation of a highly asymmetric F 0 F 1 ATP synthase complex from Micrococcus luteus into polymer‐supported membranes. Dynamic light scattering and cryo electron microscopy confirm that the use of weak surfactants (bile acid) allows for the non‐disruptive protein incorporation into lipid vesicles. Spreading of vesicles with ATP synthase onto a cellulose support results in a homogeneous distribution of proteins, in contrast to a patchy image observed on bare glass slides. The orientation of ATP synthase can be identified using an antibody to the ATP binding site as well as from topographic profiles of the surface. The method to “align” transmembrane proteins in supported membranes would open a possibility to quantify protein functions in biomimetic model systems. magnified image
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.200800128