Activation of poly[N-(2-hydroxypropyl)methacrylamide] for the binding of bioactive molecules, 1. Activation with 4-nitrophenyl chloroformate

Proper reaction conditions were determined for the activation of water‐soluble, biocompatible poly[N‐(2‐hydroxypropyl)methacrylamide] (1) by esterification with 4‐nitrophenyl chloroformate (2). The rate constants of hydrolysis and aminolysis of the 4‐nitrophenyl ester groups in resulting polymer 3 w...

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Published in:Die Makromolekulare Chemie 1983-07, Vol.184 (7), p.1339-1344
Main Authors: Lääne, Ants, Haga, Mati, Aaviksaar, Aavo, Chytrý, Vladimír, Kopeček, Jindřich
Format: Article
Language:English
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Summary:Proper reaction conditions were determined for the activation of water‐soluble, biocompatible poly[N‐(2‐hydroxypropyl)methacrylamide] (1) by esterification with 4‐nitrophenyl chloroformate (2). The rate constants of hydrolysis and aminolysis of the 4‐nitrophenyl ester groups in resulting polymer 3 were compared. It was shown that at pH 8 – 9 the rate of aminolysis in water solutions is high enough for binding bioactive molecules via NH2 groups. Cobra venom acetylcholinesterase was bound to the activated polymer 3 and thermostability and immunogenicity of this product were studied.
ISSN:0025-116X
0025-116X
DOI:10.1002/macp.1983.021840702