DNA repair gene O 6 ‐methylguanine‐DNA methyltransferase: Promoter hypermethylation associated with decreased expression and G:C to A:T mutations of p53 in brain tumors

The DNA repair enzyme O 6 ‐methylguanine‐DNA methyltransferase (MGMT) removes alkylating adducts from the O 6 position of guanine and protects cells from cytotoxic and mutagenic effects. Expression of MGMT is decreased in some cancers, which may be the result of methylation of CpG islands of both th...

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Published in:Molecular carcinogenesis 2003-01, Vol.36 (1), p.23-31
Main Authors: Yin, Dong, Xie, Dong, Hofmann, Wolf‐Karsten, Zhang, Wenxuan, Asotra, Kamlesh, Wong, Rex, Black, Keith L, Koeffler, H. Phillip
Format: Article
Language:English
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Summary:The DNA repair enzyme O 6 ‐methylguanine‐DNA methyltransferase (MGMT) removes alkylating adducts from the O 6 position of guanine and protects cells from cytotoxic and mutagenic effects. Expression of MGMT is decreased in some cancers, which may be the result of methylation of CpG islands of both the promoter and coding regions of the gene. We studied the methylation status of the MGMT promoter in a very large collection of brain tumors (85) using methylation‐specific polymerase chain reaction (PCR). Aberrant methylation occurred in 48% of 85 human brain tumor samples. Quantitative real‐time PCR showed that expression of MGMT mRNA levels was significantly decreased ( P  
ISSN:0899-1987
1098-2744
DOI:10.1002/mc.10094