Cytotoxicity of Maillard reaction products determined with a peptide spot library

The reaction of reactive carbonyl compounds (RCCs) with lysine and arginine (Maillard reaction) is a common modification of proteins in thermally processed foods. In this study, the toxicity of Maillard reaction products (MRPs) formed from defined amino acids or dipeptides (bound to a cellulose memb...

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Bibliographic Details
Published in:Molecular nutrition & food research 2009-08, Vol.53 (8), p.1019-1029
Main Authors: Muscat, Sonja, Pischetsrieder, Monika, Maczurek, Annette, Rothemund,, Sven, Münch, Gerald
Format: Article
Language:English
Subjects:
Amino Acids - toxicity
Animals
Biological and medical sciences
Cell Adhesion
Cell Proliferation
Cell Survival - drug effects
Cell viability
Cells, Cultured
Dipeptides - toxicity
Food industries
Fundamental and applied biological sciences. Psychology
Glycation
Maillard products
Maillard Reaction
Mice
Peptide Library
Proliferation
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Summary:The reaction of reactive carbonyl compounds (RCCs) with lysine and arginine (Maillard reaction) is a common modification of proteins in thermally processed foods. In this study, the toxicity of Maillard reaction products (MRPs) formed from defined amino acids or dipeptides (bound to a cellulose membrane) with ribose, glycerinaldehyde or methylglyoxal was investigated. Murine RAW 264.7 macrophages were cultivated on the cellulose membrane and the effect of MRPs on cell viability was determined. The toxicity of MRPs was dependent on the RCC used and increased in the order of ribose < glycerinaldehyde < methylglyoxal. The dipeptides were more cytotoxic than the amino acids, with Lys-Lys MRPs being the most toxic of all tested MRPs. Cell numbers did not fall below the starting point, indicating that the MRPs rather inhibited proliferation than actually caused cell death. To develop an assay, in which whole membranes with multiple peptide spots could be tested simultaneously, we measured cell numbers on larger cellulose membranes using image analysis of the intracellularly formed formazan crystals. Although this method was technically feasable, it appears that uneven cell attachment on the membrane would require a way to detemine starting cell number by a non-destructive assay to yield more robust data.
ISSN:1613-4125
1613-4133
DOI:10.1002/mnfr.200800366