ADAM7 (a disintegrin and metalloprotease 7) mRNA is suppressed in mouse epididymis by neonatal exposure to Diethylstilbestrol

• Published in: Molecular reproduction and development 2003-04, Vol.64 (4), p.414-421
• Main Authors: Adachi, Tetsuya, Matsuno, Yoshiharu, Sugimura, Atsushi, Takano, Kaiya, Koh, Kyu-Bom, Sakurai, Kenichi, Shibayama, Takako, Iguchi, Taisen, Mori, Chisato, Komiyama, Masatoshi
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blotting, Northern; CDNA; diethylstilbestrol; Diethylstilbestrol - pharmacology; Disintegrins - genetics; Disintegrins - metabolism; Drug toxicity and drugs side effects treatment; epididymis; Epididymis - drug effects; Epididymis - metabolism; estrogen; Estrogens, Non-Steroidal - pharmacology; in situ hybridization; Male; Medical sciences; Metalloendopeptidases - genetics; Metalloendopeptidases - metabolism; Mice; Mice, Inbred ICR; mouse; Pharmacology. Drug treatments; subtraction; Suppression, Genetic - genetics; Toxicity: urogenital system
• ISSN: 1040-452X; 1098-2795
• DOI: 10.1002/mrd.10235

We investigated whether neonatal exposure to diethylstilbestrol (DES) induces the alteration of mRNA expression in adult mouse epididymis, which plays an important role in sperm maturation. Using a cDNA subtraction method, we isolated 15 changed gene clones in neonatally DES‐treated mouse epididymides, and we found a clone homologous with a disintegrin and metalloprotease (ADAM) 7 in the epididymis, as a suppressed gene, by means of neonatal DES treatment in 8‐week‐old mice. Indeed, it was confirmed by Northern blot analysis that the ADAM7 mRNA expression in the epididymis was at a lower level in neonatally DES‐treated mice than in non‐treated mice. Moreover, in situ hybridization analysis and real‐time reverse transcription and polymerase chain reaction (real‐time RT‐PCR) revealed that ADAM7 expression was markedly reduced in the corpus region of the epididymis of DES‐treated mice as compared with non‐treated mice. Our results suggest that neonatal exposure to DES leads to the suppression of ADAM7 expression in the epididymis in the long term. ADAM7 gene expression might be a biological marker of fetal or neonatal exposure to estrogenic compounds, including endocrine disruptors. Mol. Reprod. Dev. 64: 414–421, 2003. © 2003 Wiley‐Liss, Inc.