Staphylococcal α-toxin causes increased tracheal epithelial permeability

Staphylococcus aureus is an important cause of pulmonary infections. The role of S. aureus α‐toxin as a virulence factor is unclear. We hypothesized that airway epithelium is a target of S. aureus α‐toxin and that exposure of airway epithelium to α‐toxin results in damage to the airway epithelium. T...

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Bibliographic Details
Published in:Pediatric pulmonology 2006-12, Vol.41 (12), p.1146-1152
Main Authors: Phillips, James R., Tripp, Timothy J., Regelmann, Warren E., Schlievert, Patrick M., Wangensteen, O. Douglas
Format: Article
Language:English
Subjects:
airway
Animals
Bacterial diseases
Bacterial Toxins - toxicity
Biological and medical sciences
Cell Membrane Permeability - drug effects
Dextrans - pharmacokinetics
Disease Models, Animal
epithelium
Epithelium - drug effects
Epithelium - metabolism
Epithelium - pathology
Exotoxins - toxicity
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluorescein-5-isothiocyanate - pharmacokinetics
General aspects
Hemolysin Proteins - toxicity
Human bacterial diseases
Infectious diseases
Male
Medical sciences
Mice
morphometry
permeability
Pneumology
Respiratory Mucosa - drug effects
Respiratory Mucosa - metabolism
Respiratory Mucosa - pathology
Staphylococcal Infections - metabolism
Staphylococcal Infections - microbiology
Staphylococcal Infections - pathology
Staphylococcal infections, streptococcal infections, pneumococcal infections
Staphylococcus aureus
Trachea - drug effects
Trachea - metabolism
Trachea - pathology
Tracheitis - metabolism
Tracheitis - microbiology
Tracheitis - pathology
α-toxin
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Summary:Staphylococcus aureus is an important cause of pulmonary infections. The role of S. aureus α‐toxin as a virulence factor is unclear. We hypothesized that airway epithelium is a target of S. aureus α‐toxin and that exposure of airway epithelium to α‐toxin results in damage to the airway epithelium. To examine the hypothesis that α‐toxin is capable of independently producing airway epithelium damage as measured by permeability and morphometry, an isolated whole mouse trachea test apparatus was developed. In vitro epithelial permeability (P) was calculated and digital micrographs were analyzed morphometrically. Purified S. aureus α‐toxin produced a significant increase in tracheal epithelial P (P 
ISSN:8755-6863
1099-0496
DOI:10.1002/ppul.20501