Assessing the risk of resistance in Pseudoperonospora cubensis to the fungicide flumorph in vitro

• Published in: Pest management science 2008-03, Vol.64 (3), p.255-261
• Main Authors: Zhu, Shusheng, Liu, Pengfei, Liu, Xili, Li, Jianqiang, Yuan, Shankui, Si, Naiguo
• Format: Article
• Language: English
• Subjects: adaptation; azoxystrobin; Biological and medical sciences; Control; cross resistance; cucumber downy mildew; Cucumis sativus - microbiology; dimethomorph; Drug Resistance, Fungal - genetics; flumorph; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; fungicide resistance; Fungicides, Industrial - toxicity; in vitro studies; Methacrylates - toxicity; Morpholines - toxicity; mutagenesis; mutants; Mutation; oomycete fungicides; Oomycetes - drug effects; Oomycetes - genetics; Oomycetes - growth & development; Oomycetes - pathogenicity; pathogenicity; Phytopathology. Animal pests. Plant and forest protection; Plant Leaves - microbiology; Pseudoperonospora cubensis; Pyrimidines - toxicity; risk assessment; Selection, Genetic; sporulation; strains; Strobilurins; ultraviolet radiation; Ultraviolet Rays
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.1515

BACKGROUND: The oomycete fungicide flumorph is a recently introduced carboxylic acid amide (CAA) fungicide. In order to evaluate the risk of developing field resistance to flumorph, the authors compared it with dimethomorph and azoxystrobin with respect to the ease of obtaining resistant isolates to these fungicides, the level of resistance and their fitness in the laboratory.RESULTS: Mutants with a high level of resistance to azoxystrobin were isolated readily by adaptation and UV irradiation, and their fitness was as good as that of the parent isolates. Attempts to generate mutants of Pseudoperonospora cubensis (Burk. & MA Curtis) Rostovsev resistant to flumorph and dimethomorph by sporangia adaptation on fungicide-treated leaves were unsuccessful. However, moderately resistant mutants were isolated using UV mutagenesis, but their resistance level [maximum resistance factor (MRF) < 100] was much lower than that of the azoxystrobin-resistant mutant (MRF = 733). With the exception of stability of resistance, all mutants showed low pathogenicity and sporulation compared with wild-type isolates and azoxystrobin-resistant mutants. There is cross-resistance between flumorph and dimethomorph, suggesting that they have the same resistance mechanism.CONCLUSION: The above results suggest that the resistance risk of flumorph may be similar to that of dimethomorph but lower than that of azoxystrobin and can be classified as moderate. Thus, it can be managed by appropriate product use strategies. Copyright © 2007 Society of Chemical Industry