Effects of methanol extract of Cirsium japonicum var. ussuriense and its principle, hispidulin-7-O-neohesperidoside on hepatic alcohol-metabolizing enzymes and lipid peroxidation in ethanol-treated rats

• Published in: Phytotherapy research 2004-01, Vol.18 (1), p.19-24
• Main Authors: Park, Jong Cheol, Hur, Jong Moon, Park, Ju Gwon, Kim, Sang Cheol, Park, Jeong Ro, Choi, Seong Hee, Choi, Jong Won
• Format: Article
• Language: English
• Subjects: Administration, Oral; alcohol detoxification; alcohol-metabolizing enzyme; Animals; Biological and medical sciences; Chemical and Drug Induced Liver Injury - enzymology; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - prevention & control; Cirsium; Cirsium japonicum var. ussuriense; Compositae; Dipeptides - drug effects; Ethanol; Flavones; Flavonoids - administration & dosage; Flavonoids - pharmacology; Flavonoids - therapeutic use; General pharmacology; Glutathione Reductase - drug effects; hispidulin 7-O-neohesperidoside; lipid peroxidation; Lipid Peroxidation - drug effects; Medical sciences; Mitochondria, Liver - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytotherapy; Plant Components, Aerial; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Protective Agents - administration & dosage; Protective Agents - pharmacology; Protective Agents - therapeutic use; Rats; Rats, Sprague-Dawley; Thiobarbituric Acid Reactive Substances
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.1299

Effects of the methanol extract of Cirsium japonicum var. ussuriense and hispidulin 7‐O‐neohesperidoside isolated from the plant on hepatic alcohol‐metabolizing enzymes and lipid peroxidation were studied in rats treated with ethanol. Rats treated with 10% alcohol solution for 6 weeks were orally administered with 250 or 500 mg of methanol extract or 10 or 20 mg of hispidulin 7‐O‐neohesperidoside per kg body weight daily during the last week of ethanol treatment. The administration of the methanol extract of herbal plant and hispidulin 7‐O‐neohesperidoside in ethanol‐treated rats significantly enhanced the activities of hepatic alcohol dehydrogenase, microsomal ethanol‐oxidizing system and aldehyde dehydrogenase in a dose‐dependent manner. The extract and the compound decreased hepatic lipid peroxidation along with an increase in hepatic content of reduced glutathione. The methanol extract and hispidulin 7‐O‐neohesperidoside of C. japonicum var. ussuriense also increased the activity of glutathione reductase, but had no effect on γ‐glutamylcysteine synthase. The results suggest that C. japonicum var. ussuriense may alleviate alcoholic toxicity by enhancing ethanol oxidation as well as inhibiting lipid peroxidation, and hispidulin 7‐O‐neohesperidoside is one of the active substances responsible for the protective effects of this plant. Copyright © 2004 John Wiley & Sons, Ltd.