Antimutagenic in vitro activity of plant polyphenols: Pycnogenol® and Ginkgo biloba extract (EGb 761)

Ofloxacin (15 μg/mL) and acridine orange (5 μg/mL) induce mutagenicity by different mechanisms in the photosynthetic flagellate Euglena gracilis. The present study examined whether Pycnogenol® (PYC; 5-100 μg/mL) or Ginkgo biloba extract (EGb 761; 5-100 μg/mL) could protect against the mutagenic effe...

Full description

Saved in:
Bibliographic Details
Published in:Phytotherapy research 2008-03, Vol.22 (3), p.384-388
Main Authors: KRIZKOVA, Livia, CHOVANOVA, Zuzana, DURACKOVA, Zdenka, KRAJCOVIC, Juraj
Format: Article
Language:English
Subjects:
acridine orange
Acridine Orange - metabolism
Acridine Orange - pharmacology
Animals
Antimutagenic Agents - pharmacology
antimutagenicity
Biological and medical sciences
Cattle
DNA - drug effects
Euglena gracilis - drug effects
Flavonoids - metabolism
Flavonoids - pharmacology
General pharmacology
Ginkgo biloba extract (EGb 761)
Medical sciences
Models, Animal
Mutagens - pharmacology
ofloxacin
Ofloxacin - pharmacology
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - metabolism
Plant Extracts - pharmacology
Pycnogenol
Spectrophotometry, Ultraviolet
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ofloxacin (15 μg/mL) and acridine orange (5 μg/mL) induce mutagenicity by different mechanisms in the photosynthetic flagellate Euglena gracilis. The present study examined whether Pycnogenol® (PYC; 5-100 μg/mL) or Ginkgo biloba extract (EGb 761; 5-100 μg/mL) could protect against the mutagenic effects of each of the mutagens and the potential mechanisms underlying such protection. The highest concentration of PYC and EGb 761 effectively reduced the mutagenic activity of both ofloxacin and acridine orange by more than 99% (p < 0.001). Using luminol-dependent photochemical methodology it was demonstrated that EGb 761 and PYC were effective antioxidants. In addition, as determined by spectrophotometry, PYC and EGb 761 bound acridine orange. Both PYC and EGb 761 have been shown to produce dual antimutagenic effects, as evidenced by both antioxidant and physicochemical properties. The findings suggest that EGb 761 and PYC would thus be suitable for future study, not only as antioxidants, but also as antimutagenic agents. Copyright © 2007 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.2331