A methoxylated fatty acid isolated from the brown seaweed Ishige okamurae inhibits bacterial phospholipase A 2

A methoxylated fatty acid that inhibits phospholipase A 2 (PLA 2 ; EC 3.1.1.4) was purified from the brown seaweed Ishige okamurae . Approximately 8.1 mg of the inhibitory compound, 7‐methoxy‐9‐methylhexadeca‐4,8‐dienoic acid, was isolated from 1 kg of I . okamurae powder. Recombinant PLA 2 derived...

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Published in:Phytotherapy research 2008-08, Vol.22 (8), p.1070-1074
Main Authors: Cho, Ji‐Young, Gyawali, Yadav P., Ahn, Sun‐Hee, Khan, Mohammed N. A., Kong, In‐Soo, Hong, Yong‐Ki
Format: Article
Language:English
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Summary:A methoxylated fatty acid that inhibits phospholipase A 2 (PLA 2 ; EC 3.1.1.4) was purified from the brown seaweed Ishige okamurae . Approximately 8.1 mg of the inhibitory compound, 7‐methoxy‐9‐methylhexadeca‐4,8‐dienoic acid, was isolated from 1 kg of I . okamurae powder. Recombinant PLA 2 derived from the pathogenic bacterium Vibrio mimicus was used as the target enzyme. The methoxylated fatty acid compound competitively inhibited PLA 2 with a Ki value of 3.9 µg/mL. The concentrations required for 50% inhibition of PLA 2 , oedema and erythema were 1.0 µg/mL, 3.6 mg/mL and 4.6 mg/mL, respectively. The compound strongly inhibited PLA 2 activity in vitro and had potent antiinflammatory activity in vivo . Copyright © 2008 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.2456