Andrographis paniculata (Ness) induces relaxation of uterus by blocking voltage operated calcium channels and inhibits Ca+2 influx

The possible relaxation of uterine smooth muscle by Andrographis paniculata dried extract via a blockade of voltage operated calcium channels was investigated in rats. Uterine horns pretreated with oestradiol were incubated in Ca+2‐free Jalon's solution and stimulated with KCl (20–60 mM) in ord...

Full description

Saved in:
Bibliographic Details
Published in:Phytotherapy research 2001-05, Vol.15 (3), p.235-239
Main Authors: Burgos, Rafael A., Aguila, Marcela J., Santiesteban, Elson. T., Sánchez, Nury S., Hancke, Juan L.
Format: Article
Language:English
Subjects:
45Ca+2
acetylcholine
Andrographis paniculata
Biological and medical sciences
calcium channels
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
smooth muscle
uterus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The possible relaxation of uterine smooth muscle by Andrographis paniculata dried extract via a blockade of voltage operated calcium channels was investigated in rats. Uterine horns pretreated with oestradiol were incubated in Ca+2‐free Jalon's solution and stimulated with KCl (20–60 mM) in order to produce depolarization of the membrane. The isometric contractile response to 1 mM or cumulative concentrations of CaCl2 were blockaded by 0.2, 0.4 and 0.8 mg/mL of A. paniculata. The maximum contractile response induced by acetylcholine was moderately antagonized by A. paniculata. The possible blockade of Ca+2 entry by A. paniculata was evaluated with 45Ca+2 uptake in uterine rings incubated with free‐Ca+2‐Ringer's solution high in K+ (KCl 40 mM). The influx was completely blockaded with 0.4 mg/mL of A. paniculata. These results strongly suggest that A. paniculata blockades voltage operated calcium channels inhibiting the entry of Ca+2 from the external medium. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.721