δ 15 N of soil N and plants in a N‐saturated, subtropical forest of southern China

• Published in: Rapid communications in mass spectrometry 2010-09, Vol.24 (17), p.2499-2506
• Main Authors: Koba, K., Isobe, K., Takebayashi, Y., Fang, Y.T., Sasaki, Y., Saito, W., Yoh, M., Mo, J., Liu, L., Lu, X., Zhang, T., Zhang, W., Senoo, K.
• Format: Article
• Language: English
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/rcm.4648

We investigated the δ 15 N profile of N (extractable NH , NO , and organic N (EON)) in the soil of a N‐saturated subtropical forest. The order of δ 15 N in the soil was EON > NH  > NO . Although the δ 15 N of EON had been expected to be similar to that of bulk soil N, it was higher than that of bulk soil N by 5‰. The difference in δ 15 N between bulk soil N and EON (Δ 15 N bulk‐EON ) was correlated significantly with the soil C/N ratio. This correlation implies that carbon availability, which determines the balance between N assimilation and dissimilation of soil microbes, is responsible for the high δ 15 N of EON, as in the case of soil microbial biomass δ 15 N. A thorough δ 15 N survey of available N (NH , NO , and EON) in the soil profiles from the organic layer to 100 cm depth revealed that the δ 15 N of the available N forms did not fully overlap with the δ 15 N of plants. This mismatch in δ 15 N between that of available N and that of plants reflects apparent isotopic fractionation during N uptake by plants, emphasizing the high N availability in this N‐saturated forest. Copyright © 2010 John Wiley & Sons, Ltd.