Therapeutic Nanozyme: Antioxidative and cytoprotective effects of nanoceria against hydrogen peroxide induced oxidative stress in fibroblast cells and in zebrafish

• Published in: ChemistrySelect (Weinheim) 2016-07, Vol.1 (11), p.2849-2856
• Main Authors: Bhushan, Bharat, Nandhagopal, Soundharapandiyan, Kannan, Rajaretinam Rajesh, Gopinath, P.
• Format: Article
• Language: English
• Subjects: Fibroblast cells; Nanoceria, Antioxidant; Oxidative stress; Zebrafish
• ISSN: 2365-6549; 2365-6549
• DOI: 10.1002/slct.201600736

Biomimetic nanoparticles emerge as an auspicious class of antioxidative nano‐agents owing to their inherent catalytic properties. Among them, nanoceria revolutionizes the field of nanomedicine due to its astonishing antioxidant enzyme mimetic activities. However, the complete pharmacological potential of nanoceria needs to be explored yet. In this work, antioxidant and cytoprotective potentials of nanoceria against H2O2 induced oxidative stress was examined both in fibroblast cells and in zebrafish. Furthermore, the cellular uptake, cell cycle, mitochondrial membrane potential, morphological and nuclear analysis suggests that the nanoceria successfully defend the cells against oxidative stress induced cell death. Thus, the present study opens up new avenue of nanoceria for future biomedical applications. The biocompatibility and protective effect of nanoceria against H2O2 induced damage was assessed both in vitro and in vivo in fibroblast cells and zebrafish, respectively. The antioxidant potential of nanoceria has been realized for the first time against zebrafish, which could be utilized as a valuable laboratory alternative in vivo model for future antioxidant experiments.