Electrochemical Determination of Tinidazole and Brucine Using Poly (l‐Glutamic Acid) Modified Carbon Nanotube Paste Electrode

• Published in: ChemistrySelect (Weinheim) 2024-11, Vol.9 (41), p.n/a
• Main Authors: Raksha, B., Manjunatha, J. G., Sumanth, D., Kumar, Naveen, Aldossari, S. A., Raril, C.
• Format: Article
• Language: English
• Subjects: Analysis; Brucine; Carbon nanotube; l‐Glutamic acid; Real sample; Sensing; Tinidazole
• ISSN: 2365-6549; 2365-6549
• DOI: 10.1002/slct.202404341

This study developed an electrochemical method for the selective and sensitive detection of Tinidazole (TZ) by modifying a bare carbon nanotube paste electrode (BCNTPE) with electrochemically polymerized l‐Glutamic acid (P(L‐GA)/MCNTPE). The modified carbon nanotube paste electrode (MCNTPE) achieved optimal detection with an irreversible peak in a 0.2 M phosphate buffer solution (PBS) at pH 3.5. Characterization of both the P(L‐GA)/MCNTPE and BCNTPE was carried out using techniques such as scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and linear sweep voltammetry (LSV). The sensor effectively identified possible interferences from different metals and organic compounds, with scan rate studies indicating that the reaction was controlled by diffusion. The effects of pH and concentration variations were also thoroughly investigated. The active surface area of the P(l‐GA)/MCNTPE and BCNTPE were found to be 0.471 cm2 and 0.217 cm2 respectively. The P(l‐GA)/MCNTPE displayed excellent electrochemical properties, with a limit of detection (LOD) of 0.06 µM and a limit of quantification (LOQ) of 0.2 µM, along with good reproducibility, repeatability, and stability. This study presents a P(l‐GA) MCNTPE that significantly enhances the sensitivity and selectivity for simultaneous detection of TZ and bare carbon nanotube (BCN). The improved electroactive surface area and electron transfer dynamics demonstrate its potential for practical applications in pharmaceutical analysis. The current study demonstrates the detection of Tinidazole (TZ) using polymerized L‐glutamic acid (P(l‐GA)/MCNTPE. The graphical outlines the process of preparing P(l‐GA)/MCNTPE, starting with the fabrication of a carbon nanotube paste. This paste is stuffed into the cavity of a Teflon tube, resulting in the formation of BCNTPE. Next, the BCNTPE is modified by electropolymerizing l‐glutamic acid (l‐GA) onto it, producing P(l‐GA)/MCNTPE. The finalized electrode is then utilized for the voltametric analysis of TZ, and its application extends to the investigation of pharmaceutical samples.