Design, Assembly, and Activity of Antisense DNA Nanostructures

Discrete DNA nanostructures allow simultaneous features not possible with traditional DNA forms: encapsulation of cargo, display of multiple ligands, and resistance to enzymatic digestion. These properties suggested using DNA nanostructures as a delivery platform. Here, DNA pyramids displaying antis...

Full description

Saved in:
Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2011-12, Vol.7 (24), p.3529-3535
Main Authors: Keum, Jung-Won, Ahn, Jin-Ho, Bermudez, Harry
Format: Article
Language:English
Subjects:
Animals
antisense regulation
DNA nanotechnology
DNA, Antisense - chemical synthesis
DNA, Antisense - chemistry
DNA, Antisense - metabolism
Flow Cytometry
gene therapy
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Mice
Nanostructures - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
self-assembly
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Discrete DNA nanostructures allow simultaneous features not possible with traditional DNA forms: encapsulation of cargo, display of multiple ligands, and resistance to enzymatic digestion. These properties suggested using DNA nanostructures as a delivery platform. Here, DNA pyramids displaying antisense motifs are shown to be able to specifically degrade mRNA and inhibit protein expression in vitro, and they show improved cell uptake and gene silencing when compared to linear DNA. Furthermore, the activity of these pyramids can be regulated by the introduction of an appropriate complementary strand. These results highlight the versatility of DNA nanostructures as functional devices. The unique properties of DNA nanostructures suggested their use as a gene/drug delivery platform. DNA pyramids displaying antisense motifs can specifically degrade mRNA and inhibit protein expression in vitro, and show improved cell uptake and gene silencing when compared to linear DNA. Furthermore, the activity of these pyramids can be regulated by the introduction of an appropriate complementary strand. The results highlight the versatility of DNA nanostructures as functional devices.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201101804