Data‐Matrix Technology for Multiparameter Monitoring of Cell Cultures

A data‐matrix (DM)‐technology approach in cell biology is implemented as an efficient method for the multiparameter monitoring of cell cultures. The proposed method takes advantage of the know‐how developed for fault tolerance in digital information technology by measuring the amount of errors induc...

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Bibliographic Details
Published in:Small methods 2018-04, Vol.2 (4), p.n/a
Main Authors: Barbalinardo, Marianna, Gentili, Denis, Lazzarotto, Francesca, Valle, Francesco, Brucale, Marco, Melucci, Manuela, Favaretto, Laura, Zambianchi, Massimo, Borrachero‐Conejo, Ana I., Saracino, Emanuela, Benfenati, Valentina, Natalini, Davide, Greco, Paolo, Di Carlo, Maria Giovanna, Foschi, Giulia, Cavallini, Massimiliano
Format: Article
Language:English
Subjects:
cell culture devices
data matrices
fabrication
patterning
soft lithography
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Summary:A data‐matrix (DM)‐technology approach in cell biology is implemented as an efficient method for the multiparameter monitoring of cell cultures. The proposed method takes advantage of the know‐how developed for fault tolerance in digital information technology by measuring the amount of errors induced by intervening cells upon checking a DM code placed behind them. It gives continuous access to several quantitative parameters of the observed culture, such as cell coverage, mean size, viability, and transfection efficiency. Data‐matrix technology in life science is introduced as an efficient method for multiparameter cell culture monitoring. The method counts the errors induced by intervening cells upon checking a data‐matrix code placed behind them for measuring number of cells and coverage in real time. It is demonstrated with several model cell cultures and to measure the transfection efficiency.
ISSN:2366-9608
2366-9608
DOI:10.1002/smtd.201700377