Polyprotein Assembly: Mechanical Polyprotein Assembly Using Sfp and Sortase‐Mediated Domain Oligomerization for Single‐Molecule Studies (Small Methods 6/2018)

Polyproteins are multidomain protein oligomers used for single‐molecule biomechanical experiments on protein‐folding energy landscapes. In article number 1800039, Michael A. Nash and co‐workers demonstrate a new method for posttranslational polyprotein synthesis using an enzyme‐mediated process. The...

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Bibliographic Details
Published in:Small methods 2018-06, Vol.2 (6), p.n/a
Main Authors: Liu, Haipei, Ta, Duy Tien, Nash, Michael A.
Format: Article
Language:English
Subjects:
atomic force microscopy
cohesion and dockerin
polyproteins
protein folding
single‐molecule force spectroscopy
sortase
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Summary:Polyproteins are multidomain protein oligomers used for single‐molecule biomechanical experiments on protein‐folding energy landscapes. In article number 1800039, Michael A. Nash and co‐workers demonstrate a new method for posttranslational polyprotein synthesis using an enzyme‐mediated process. The method allows single‐molecule folding and unfolding studies on protein domains that are insoluble or unfoldable when expressed in vivo as a polyprotein.
ISSN:2366-9608
2366-9608
DOI:10.1002/smtd.201870033