DNA binding in combination with capillary electrophoresis and inductively coupled plasma mass spectrometry for the rapid speciation analysis of mercury

We herein developed a method for the rapid speciation analysis of Hg2+ and CH3Hg+ by using specific DNA sequences to bind Hg2+ and CH3Hg+ together with capillary electrophoresis and inductively coupled plasma mass spectrometry. The specific DNA sequences can combine with Hg2+ and CH3Hg+ with differe...

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Bibliographic Details
Published in:Separation science plus 2018-01, Vol.1 (1), p.52-58
Main Authors: Chen, Yiquan, Chen, Lian, Wang, Xusheng, Xi, Zhiming, Wu, Yongning, Fu, FengFu
Format: Article
Language:English
Subjects:
aptamers
inorganic mercury
methyl mercury
organic mercury
seafood
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Summary:We herein developed a method for the rapid speciation analysis of Hg2+ and CH3Hg+ by using specific DNA sequences to bind Hg2+ and CH3Hg+ together with capillary electrophoresis and inductively coupled plasma mass spectrometry. The specific DNA sequences can combine with Hg2+ and CH3Hg+ with different binding force, which makes the formed DNA‐Hg2+ and DNA‐CH3Hg+ conjugates possess different negative charges, and thus make Hg2+ and CH3Hg+ can be more easily and rapidly separated by capillary electrophoresis under reverse mode. Under the optimal conditions, the Hg2+ and CH3Hg+ can be baseline separated and detected by capillary electrophoresis‐inductively coupled plasma mass spectrometry within 11 min with a detection limit of 0.12 and 0.10 ng/mL, respectively. With the help of the method, we have successfully determined Hg2+ and CH3Hg+ in dried fish muscle samples with a recovery of 95–101% and a relative standard deviation (n = 6)
ISSN:2573-1815
2573-1815
DOI:10.1002/sscp.201700015