α2-8 Sialic Acid Polymers: Size, Structure, and Compositional Analysis

A series of three variable assay procedures is described to provide overlapping information on the size, structure, and composition of the α2-8 linked polysialic acid chains present on a wide variety of critical cell surface glycoproteins. Technical advances in instrumentation have permitted the dev...

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Bibliographic Details
Published in:Analytical biochemistry 1994-11, Vol.222 (2), p.495-502
Main Authors: Ashwell, G., Berlin, W.K., Gabriel, O.
Format: Article
Language:English
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Summary:A series of three variable assay procedures is described to provide overlapping information on the size, structure, and composition of the α2-8 linked polysialic acid chains present on a wide variety of critical cell surface glycoproteins. Technical advances in instrumentation have permitted the development of new applications for a methodology involving the sequential use of periodate and borohydride to modify terminal sialic acid residues. The procedures described here provide a rapid and facile assay for (a) the determination of polysialic acid chain length, (b) the simultaneous identification of N-acetylneuraminic acid, N-glycolylneuraminic acid, and KDN (deaminated sialic acid) when present in a single preparation, (c) the ability to distinguish qualitatively between reducing and nonreducing polymers, and (d) the ability to determine the number of chains bound to a glycoprotein of known molecular weight.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1994.1523