Identification of Hydrazine in Commercial Preparations of Carnosine and Its Influence on Carnosine's Antioxidative Properties

Commercial preparations of synthetic carnosine are commonly used by researchers to investigate carnosine's biological functions and potential applications. Our studies on the interaction of synthetic carnosine and aldehydic lipid oxidation products have led to the detection and structural ident...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1998-07, Vol.261 (1), p.79-86
Main Authors: Zhou, Shengying, Dickinson, L.Charles, Yang, Li, Decker, Eric A.
Format: Article
Language:English
Subjects:
Aldehydes - analysis
Amino Acids - isolation & purification
Antioxidants - isolation & purification
Antioxidants - pharmacology
Carnosine - isolation & purification
Carnosine - pharmacology
Chemistry Techniques, Analytical
Chromatography, High Pressure Liquid
Drug Contamination
Gas Chromatography-Mass Spectrometry
Hydrazines - analysis
Hydrazines - pharmacology
Lipid Peroxidation - drug effects
Liposomes
Magnetic Resonance Spectroscopy
Malondialdehyde - analysis
Oligopeptides - isolation & purification
Thiobarbituric Acid Reactive Substances - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Commercial preparations of synthetic carnosine are commonly used by researchers to investigate carnosine's biological functions and potential applications. Our studies on the interaction of synthetic carnosine and aldehydic lipid oxidation products have led to the detection and structural identification of hydrazine, a strong reducing agent. The concentrations of hydrazine in various sources of commercial carnosine were in the range of 0.01–0.20% (w/w). The levels of contaminating hydrazine in commercial carnosine were capable of interfering with the analyses of headspace aldehydes, malonaldehyde, and thiobarbituric acid-reactive substances. Since hydrazine can potentially interfere with lipid oxidation reactions and measurement of lipid oxidation products, it will be necessary to use purified carnosine to reevaluate carnosine's biological and chemical properties.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1998.2724