Simultaneous Determination of N-Acetylaspartic Acid, N-Acetylglutamic Acid, and N-Acetylaspartylglutamic Acid in Whole Brain of 3-Mercaptopropionic Acid-Treated Rats Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

The measurement of N-acetylaspartic acid (NAA), N-acetylglutamic acid (NAG), and N-acetylaspartylglutamic acid (NAAG) in the whole brain of 3-mercaptopropionic acid (3-MPA)-treated rats has been developed using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface...

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Published in:Analytical biochemistry 1999-12, Vol.276 (2), p.124-128
Main Authors: Ma, Deshun, Zhang, Jianying, Sugahara, Kazunori, Ageta, Tomiko, Nakayama, Kazuko, Kodama, Hiroyuki
Format: Article
Language:English
Subjects:
3-mercaptopropionic acid
3-Mercaptopropionic Acid - pharmacology
Animals
Aspartic Acid - analogs & derivatives
Aspartic Acid - analysis
Atmospheric Pressure
brain
Brain - drug effects
Brain Chemistry
Chromatography, Liquid
Dipeptides - analysis
Enzyme Inhibitors - pharmacology
Glutamate Decarboxylase - antagonists & inhibitors
Glutamates - analysis
LC-APCI-MS
Male
Mass Spectrometry - methods
N-acetylaspartic acid
N-acetylaspartylglutamic acid
N-acetylglutamic acid
Rats
Rats, Wistar
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cited_by cdi_FETCH-LOGICAL-c340t-4a7c98116fc3611a48b52f3638932625c99b3df763723e14ca01b53bf7ebb7443
cites cdi_FETCH-LOGICAL-c340t-4a7c98116fc3611a48b52f3638932625c99b3df763723e14ca01b53bf7ebb7443
container_end_page 128
container_issue 2
container_start_page 124
container_title Analytical biochemistry
container_volume 276
creator Ma, Deshun
Zhang, Jianying
Sugahara, Kazunori
Ageta, Tomiko
Nakayama, Kazuko
Kodama, Hiroyuki
description The measurement of N-acetylaspartic acid (NAA), N-acetylglutamic acid (NAG), and N-acetylaspartylglutamic acid (NAAG) in the whole brain of 3-mercaptopropionic acid (3-MPA)-treated rats has been developed using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. The recoveries of these compounds were 90.85 ± 3.43% for NAA, 91.62 ± 5.47% for NAG, and 92.29 ± 4.44% for NAAG. The detection limits for NAA, NAG, and NAAG were 12, 15, and 20 μg/ml, respectively. After administration of 3-MPA, the concentrations of NAA, NAG, and NAAG in the whole brain over 10 min increased 177.25, 134.23, and 127.70%, respectively. These concentrations then decreased over the next 60 min. The simultaneous determination of NAA, NAG, and NAAG using this method was found to be very useful for studies of metabolism of NAA, NAG, and NAAG in biological samples.
doi_str_mv 10.1006/abio.1999.4352
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subjects 3-mercaptopropionic acid
3-Mercaptopropionic Acid - pharmacology
Animals
Aspartic Acid - analogs & derivatives
Aspartic Acid - analysis
Atmospheric Pressure
brain
Brain - drug effects
Brain Chemistry
Chromatography, Liquid
Dipeptides - analysis
Enzyme Inhibitors - pharmacology
Glutamate Decarboxylase - antagonists & inhibitors
Glutamates - analysis
LC-APCI-MS
Male
Mass Spectrometry - methods
N-acetylaspartic acid
N-acetylaspartylglutamic acid
N-acetylglutamic acid
Rats
Rats, Wistar
title Simultaneous Determination of N-Acetylaspartic Acid, N-Acetylglutamic Acid, and N-Acetylaspartylglutamic Acid in Whole Brain of 3-Mercaptopropionic Acid-Treated Rats Using Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
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