A Polyomavirus Enhancer Mutant Confers Ubiquitous High Transcriptional Efficiency to the SV40 Late Promoter

To identify expression plasmids with high efficiency of transcription and with a broad tissue and cell range, we have constructed a recombinant vector combining the late SV40 promoter and the polyomavirus regulatory region derived from a mutant (PyNB11/1) which displays a very wide host range. We sh...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-02, Vol.207 (1), p.339-347
Main Authors: Fimia, G.M., Ferreira, R., Passananti, C., Amati, P.
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Base Sequence
Cell Line
Cercopithecus aethiops
Chloramphenicol O-Acetyltransferase - biosynthesis
Enhancer Elements, Genetic
Gene Expression
HeLa Cells
Humans
Liver Neoplasms
Mice
Molecular Sequence Data
Polyomavirus - genetics
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Restriction Mapping
Simian virus 40 - genetics
Teratoma
Transcription, Genetic
Transfection
Tumor Cells, Cultured
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Summary:To identify expression plasmids with high efficiency of transcription and with a broad tissue and cell range, we have constructed a recombinant vector combining the late SV40 promoter and the polyomavirus regulatory region derived from a mutant (PyNB11/1) which displays a very wide host range. We show that these recombinant enhancer-promoters are efficient drivers for heterologous gene transcription and expression in vitro in all mouse and human cells tested. The most active combination we identified contained the mutant enhancer (PyNB11/1) in the late orientation. This construct was able to promote a high efficiency of expression without significant fluctuation between cells of different tissutal origin or different differentiative stage. A possible interpretation of these results is discussed.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1193