Sequence Variation in the 5′, Putative Promoter of Sry and Its Possible Relevance to the C57BL/6J-Y-DOM Sex Reversal

Sry is the testes determining factor gene located on the Y chromosome. We sought sequence variation in potential promoter regions that might explain the gradiation in sex reversal seen when different domesticus strains are bred to C57BL/6J females since sequence variation previously found in the cod...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-03, Vol.208 (2), p.624-628
Main Authors: Graves, P.E., Erickson, R.P.
Format: Article
Language:English
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Summary:Sry is the testes determining factor gene located on the Y chromosome. We sought sequence variation in potential promoter regions that might explain the gradiation in sex reversal seen when different domesticus strains are bred to C57BL/6J females since sequence variation previously found in the coding region is not sufficient to explain it. Dideoxy sequencing identified a 10 bp deletion in the 5′ flanking region limited to YDOM chromosomes of weak effect. A PCR strategy to search for deletions in this region disclosed another deletion which was shown to be different by sequencing.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1384