Platelet-Derived Growth Factor Isoforms PDGF-AA, PDGF-AB and PDGF-BB Exert Specific Effects on Collagen Gene Expression and Mitotic Activity of Cultured Human Wound Fibroblasts

• Published in: Biochemical and biophysical research communications 1995-04, Vol.209 (2), p.393-399
• Main Authors: Lepisto, J., Peltonen, J., Vahakreula, M., Niinikoski, J., Laato, M.
• Format: Article
• Language: English
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.1516

This study was designed to clarify the action of three different isoforms of PDGF (PDGF-AA, PDGF-AB and PDGF-BB) on the proliferation rate and collagen synthesis of fibroblasts cultured from normal human wounds. PDGF-AA and PDGF-BB down-regulated both the steady-state levels of proα1(I) and proα1(III) collagen chain mRNAs and the production of collagen, both in a dose-dependent manner. Interestingly, low concentrations (1 ng/ml) of PDGF-AB up-regulated the expression of type I and III procollagen mRNAs by cultured wound fibroblasts, while under high concentrations (30 ng/ml) of PDGF-AB this effect changed to the opposite. The proliferation rate of wound fibroblasts was stimulated by PDGF-BB which elicited a dose-dependent (1-30 ng/ml) stimulation of cell proliferation, whereas PDGF-AB and -AA were less effective in this respect.