Microfilament Assembly Is Required for Anti-IgM Dependent Mapk and p90rsk Activation in Human B Lymphocytes

Mitogen-activated protein kinases (MAPK) are important mediators of signal transduction from the cell surface to the nucleus. These MAPK pathways serve different receptor-mediated signaling pathways leading to dual phosphorylation on serine/threonine and tyrosine residues. The mechanisms linking cyt...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-04, Vol.209 (3), p.1102-1110
Main Authors: Melamed, I., Franklin, R.A., Gelfand, E.W.
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - physiology
Actins - drug effects
Actins - metabolism
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Blotting, Western
Botulinum Toxins - pharmacology
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Cells, Cultured
Cytochalasin D - pharmacology
Enzyme Activation
Humans
Immunoglobulin M - immunology
Immunoglobulin M - pharmacology
Kinetics
Protein-Serine-Threonine Kinases - metabolism
Ribosomal Protein S6 Kinases
Tetradecanoylphorbol Acetate - pharmacology
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Summary:Mitogen-activated protein kinases (MAPK) are important mediators of signal transduction from the cell surface to the nucleus. These MAPK pathways serve different receptor-mediated signaling pathways leading to dual phosphorylation on serine/threonine and tyrosine residues. The mechanisms linking cytoplasmic MAPK activation to later events is still unclear. In this study we demonstrate that the microfilament system has an active role in MAPK activation. Cross-linking of surface IgM or direct activation of PKC with PMA resulted in time and concentration-dependent increases in F-actin content, MAPK (p42erk-2) activation, and phosphorylation of p90rsk. Pretreatment of the B cells with cytochalasin D or botulinum C2 toxin, microfilament-disrupting agents, prevented the increases in F-actin content as well as MAPK and p90rsk activation. These data indicate a role for the microfilament system in the complex and divergent functions of MAPK.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1611