Transcription Initiation Is Localized to a TATA-less Region in the Ovine β1 Adrenergic Receptor Gene

We cloned and sequenced the ovine beta1 adrenergic receptor (β1AR) gene and promoter region. The transcription start site was localized by RNase protection and primer extension to a GC-rich region. The predominant initiation sequence did not resemble an initiator element and there was no upstream TA...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-06, Vol.211 (1), p.254-261
Main Authors: Padbury, J.F., Tseng, Y.T., Waschek, J.A.
Format: Article
Language:English
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Summary:We cloned and sequenced the ovine beta1 adrenergic receptor (β1AR) gene and promoter region. The transcription start site was localized by RNase protection and primer extension to a GC-rich region. The predominant initiation sequence did not resemble an initiator element and there was no upstream TATA box. Sequence analysis revealed several potential thyroid hormone and glucocorticoid regulatory elements. Identification of the promoter structure of this important gene will help define its novel regulation during development.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1804