On the Relationship of the ATP-Independent, Mitochondrial-Associated DNA Topoisomerase of Saccharomyces cerevisiae to the Nuclear Topoisomerase I

A mitochondrial type I-like, ATP-independent, DNA topoisomerase, isolated from highly purified yeast mitochondria, is genetically related to nuclear topoisomerase I. We found that the mitochondrial topoisomerase activity cannot be detected in yeast mitochondrial extracts prepared from strains in whi...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-09, Vol.214 (2), p.723-729
Main Authors: Wang, J., Kearney, K., Derby, M., Wernette, C.M.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Cell Fractionation
Cell Nucleus - enzymology
DNA Topoisomerases, Type I - genetics
DNA Topoisomerases, Type I - isolation & purification
DNA Topoisomerases, Type I - metabolism
Gene Expression
Genes, Fungal
Kinetics
Mitochondria - enzymology
Mutagenesis
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Species Specificity
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Summary:A mitochondrial type I-like, ATP-independent, DNA topoisomerase, isolated from highly purified yeast mitochondria, is genetically related to nuclear topoisomerase I. We found that the mitochondrial topoisomerase activity cannot be detected in yeast mitochondrial extracts prepared from strains in which the topoisomerase I gene (TOP1) is disrupted. Thus, the topoisomerase activity associated with mitochondria is dependent upon the expression of the nuclear topoisomerase I gene.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2345