Activation of Large Conductance Ca2+-Activated K+Channels in N1E-115 Neuroblastoma Cells by Platelet-Activating Factor

Using patch-clamp single channel recording techniques, we reported a Platelet-Activating Factor “PAF”-induced activation of large conductance Ca2+-activated K+“BK(Ca)” channels in N1E-115 cells. This activation was only observed in cell-attached configuration and was blocked by the PAF antagonist BN...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-01, Vol.218 (3), p.745-748
Main Authors: Diserbo, Michel, Fatôme, Marc, Verdetti, Jean
Format: Article
Language:English
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Summary:Using patch-clamp single channel recording techniques, we reported a Platelet-Activating Factor “PAF”-induced activation of large conductance Ca2+-activated K+“BK(Ca)” channels in N1E-115 cells. This activation was only observed in cell-attached configuration and was blocked by the PAF antagonist BN50739 or removal of calcium from the bath. Nanomolar concentration of PAF produced a transient hyperpolarization observed in whole-cell current clamp configuration which was blocked by the bath application of BN50739 or iberiotoxin. Our results suggest that the PAF-induced hyperpolarization is mediated by an activation of BK(Ca) channels coupled to specific PAF receptors. This coupling is not direct and results from the PAF-induced elevation in cytosolic free Ca2+concentrations that we have previously described in N1E-115 cells (Cell Calcium1995,17,442–452).
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0132