Control of Expression of PLCβ1by LAC Repressor System: Relationship between Nuclear PLCβ1Overexpression and Growth Factor Stimulation

Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β1(...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-12, Vol.241 (1), p.122-126
Main Authors: Billi, Anna Maria, Matteucci, Alessandro, Faenza, Irene, Manzoli, Lucia, Rubbini, Silvia, Gilmour, R.Stewart, Rhee, Sue Goo, Cocco, Lucio
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin-like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C β1(PLC β1) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC β1.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.7778