Phosphorylation and Dephosphorylation of the B Subunit of DNA Polymerase α-Primase Complex in the Early Embryogenesis ofDrosophila

In the early embryos ofDrosophila,the B subunit of the DNA polymerase α-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS–polyacrylamide gel electrophoresis. Lambda phosphatase treatment showed that the reduced migration was caused...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-01, Vol.254 (2), p.372-377
Main Authors: Kuroda, Kazufumi, Ueda, Ryu
Format: Article
Language:English
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Summary:In the early embryos ofDrosophila,the B subunit of the DNA polymerase α-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS–polyacrylamide gel electrophoresis. Lambda phosphatase treatment showed that the reduced migration was caused by phosphorylation of the B subunit. Detailed analysis using the partially purified B subunit indicated that most of the B subunit until cycle 13 was a phosphorylated form while the B subunit of cycle 14 was a dephosphorylated form.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9944