Transforming Growth Factor-β1 Increases the Expression of Lectin-like Oxidized Low-Density Lipoprotein Receptor-1

• Published in: Biochemical and biophysical research communications 2000-06, Vol.272 (2), p.357-361
• Main Authors: Minami, Manabu, Kume, Noriaki, Kataoka, Hiroharu, Morimoto, Masafumi, Hayashida, Kazutaka, Sawamura, Tatsuya, Masaki, Tomoh, Kita, Toru
• Format: Article
• Language: English
• Subjects: atherosclerosis; LOX-1; oxidized low-density lipoprotein; TGF-β1; vascular remodeling
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2000.2778

Lectin-like oxidized low-density lipoprotein (Ox-LDL) receptor-1 (LOX-1) is a novel cell-surface receptor for Ox-LDL, which can be expressed by vascular endothelial cells, smooth muscle cells, and macrophages. On the other hand, transforming growth factor (TGF)-β1, which plays crucial roles in vascular remodeling and the pathogenesis of atherosclerosis, has been shown to inhibit expression of class A scavenger receptors and CD36 in macrophages. Here we provide the evidence that TGF-β1 (0.1–10 ng/mL) induces LOX-1 protein and mRNA expression in both bovine aortic endothelial cells and smooth muscle cells in a dose- and time-dependent fashion, probably at the transcriptional level. TGF-β1 also upregulates LOX-1 mRNA expression in murine peritoneal macrophages. Thus TGF-β1 can highly induce LOX-1 expression in vascular endothelial cells, smooth muscle cells, and macrophages, suggesting that TGF-β1 appears one of the key regulators that modulates expression of scavenger receptors.