Associates with a Neurite-Outgrowth-Related Protein, SFAP75

Semaphorin 4C (S4C, previously called M-SemaF) was recently identified as a brain rich transmembrane member of semaphorin family of the vertebrate. In the cytoplasmic domain of S4C there is a proline-rich region suggesting that the cytoplasmic domain may play an important role in Sema4C function. In...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001, Vol.280 (1), p.237-243
Main Authors: Ohoka, Yoshiharu, Hirotani, Mutsumi, Sugimoto, Hisako, Fujioka, Shi-ho, Furuyama, Tatsuo, Inagaki, Shinobu
Format: Article
Language:English
Subjects:
juxtamembrane
LTP
Norbin
semaphorin
Semaphorin 4C
SFAP75
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Summary:Semaphorin 4C (S4C, previously called M-SemaF) was recently identified as a brain rich transmembrane member of semaphorin family of the vertebrate. In the cytoplasmic domain of S4C there is a proline-rich region suggesting that the cytoplasmic domain may play an important role in Sema4C function. In this study, we have identified the cytoplasmic domain (cd) of M-SemaF(S4C)-associating protein with a Mr of 75 kDa, named SFAP75, from mouse brain. SFAP75 turned out to be the same as the recently reported neurite-outgrowth-related protein named Norbin. Deletion mutants analyses of S4C and SFAP75 revealed that the membrane-proximal region of S4Ccd binds to the intermediate region of SFAP75. Western blot and immunohistochemicalanalyses with anti-Sema4C and anti-SFAP75 antibodies indicated that S4C and SFAP75 were specially enriched in the brain with a similar distribution pattern to each other. These results suggest that S4C interacts with SFAP75 and plays a role in neural function in brain.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.4080