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Up-Regulation of Endothelial Nitric Oxide Synthase through β2-Adrenergic Receptor—The Role of a β-Blocker with NO-Releasing Action

We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2 receptor exists in...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-01, Vol.280 (3), p.589-594
Main Authors: Jayachandran, Muthuvel, Hayashi, Toshio, Sumi, Daigo, Thakur, Navin Kumar, Kano, Hatsuyo, Ignarro, Louis J., Iguchi, Akihisa
Format: Article
Language:English
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Summary:We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2 receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by β2 receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective β blocker on eNOS through β2 receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.4177