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Up-Regulation of Endothelial Nitric Oxide Synthase through β2-Adrenergic Receptor—The Role of a β-Blocker with NO-Releasing Action
We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2 receptor exists in...
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Published in: | Biochemical and biophysical research communications 2001-01, Vol.280 (3), p.589-594 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We determined the existence and role of β2-adrenergic receptor in cultured BAECs through the effect of a β-blocker having NO releasing action; 3,4-dihydro-8(2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. β2 receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by β2 receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective β blocker on eNOS through β2 receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1006/bbrc.2000.4177 |