Phospholipase A2-Mediated Fusion of Neutrophil-Derived Membranes Is Augmented by Phosphatidic Acid

Phosphatidic acid (PA), the product of phospholipase D (PLD) metabolism, is not only an important second messenger in neutrophil signal transduction but PA generation increases membrane fusogenicity. Following neutrophil stimulation, PA formation can be detected in azurophil, specific, and plasma me...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-03, Vol.282 (2), p.480-486
Main Authors: Harsh, Donna M., Blackwood, R.Alexander
Format: Article
Language:English
Subjects:
arachidonic acid
membrane fusion
phosphatidic acid
phospholipase A2
phospholipase D
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Summary:Phosphatidic acid (PA), the product of phospholipase D (PLD) metabolism, is not only an important second messenger in neutrophil signal transduction but PA generation increases membrane fusogenicity. Following neutrophil stimulation, PA formation can be detected in azurophil, specific, and plasma membrane vesicle subcellular fractions, suggesting a potential role for PA formation in granule–plasma membrane fusion. Neutrophil stimulation also activates phospholipase A2 (PLA2) and the release of arachidonic acid. In vitro fusion of plasma membrane vesicles and specific granules with complex liposomes were dependent on PLA2 (
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4601