Skin Surface Electric Potential Induced by Ion-Flux through Epidermal Cell Layers

The skin surface electric potential has been widely used for psychological studies because it is sensitive to emotional conditions. We measured the electric potential on the surface of hairless mice skin in organ culture with several physiological factors. Disruption of mitochondrial function and in...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-06, Vol.284 (1), p.112-117
Main Authors: Denda, Mitsuhiro, Ashida, Yutaka, Inoue, Kaori, Kumazawa, Noriyuki
Format: Article
Language:English
Subjects:
4-Aminopyridine - pharmacology
Adenosine Triphosphatases - antagonists & inhibitors
Animals
barrier
calcium
Calcium Channel Blockers - pharmacology
Chelating Agents - pharmacology
Corticotropin-Releasing Hormone - pharmacology
Enzyme Inhibitors - pharmacology
epidermis
Galvanic Skin Response - drug effects
Galvanic Skin Response - physiology
In Vitro Techniques
Ion Transport - drug effects
Ion Transport - physiology
Ionophores - pharmacology
magnesium
Male
Membrane Potentials - drug effects
Membrane Potentials - physiology
Mice
Mice, Hairless
Mitochondria - drug effects
potassium
Potassium Channel Blockers
Skin - drug effects
Skin Physiological Phenomena
Sodium Azide - pharmacology
Sodium Channel Blockers
Substance P - pharmacology
Tetrodotoxin - pharmacology
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Summary:The skin surface electric potential has been widely used for psychological studies because it is sensitive to emotional conditions. We measured the electric potential on the surface of hairless mice skin in organ culture with several physiological factors. Disruption of mitochondrial function and inhibition of ATPase reduced the skin surface potential 50–70%. Calcium, potassium, and sodium channel blockers also reduced the potential. A calcium-specific and potassium ionophore reduced the potential, but the calcium and magnesium ionophore increased it. EDTA decreased the potential but EGTA had no effect. Skin surface barrier disruption reduced the potential and calcium and potassium channel blockers partially prevented the decrease. Substance P and corticotropin-releasing factor decreased the potential, and antagonists blocked the decreases. These results suggest that the ion flux in the nucleated layer of the epidermis induce the skin surface potential and it is influenced by environmental and neuroendocrinological factors.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4925