Effects of Flow Patterns on Endothelial Cell Migration into a Zone of Mechanical Denudation

Vascular endothelial cells (ECs) in vivo are subject to different flow conditions due to the variation in vessel geometry. The aim of this study is to elucidate the effects of different flow conditions on EC monolayer migration into a mechanically denuded zone and their underlying mechanisms. Both l...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-07, Vol.285 (3), p.751-759
Main Authors: Hsu, Pin-Pin, Li, Song, Li, Yi-Shuan, Usami, Shunichi, Ratcliffe, Anthony, Wang, Xiong, Chien, Shu
Format: Article
Language:English
Subjects:
ADP Ribose Transferases - pharmacology
Animals
Aorta
Botulinum Toxins
Cattle
Cell Culture Techniques - methods
cell junction
Cell Movement - drug effects
Cell Movement - physiology
cell signaling
Cells, Cultured
disturbed flow
endothelial cell migration
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Enzyme Inhibitors - pharmacology
focal adhesion
Focal Adhesions - physiology
Genistein - pharmacology
Intercellular Junctions - physiology
laminar flow
Phosphorylation - drug effects
Rheology - instrumentation
rho GTP-Binding Proteins - antagonists & inhibitors
Stress, Mechanical
wound closure
Wound Healing - drug effects
Wound Healing - physiology
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Summary:Vascular endothelial cells (ECs) in vivo are subject to different flow conditions due to the variation in vessel geometry. The aim of this study is to elucidate the effects of different flow conditions on EC monolayer migration into a mechanically denuded zone and their underlying mechanisms. Both laminar and disturbed flows significantly enhanced EC migration. EC migration speed was the fastest under laminar flow, which preferentially promoted directional EC migration from the upstream side of the wounded monolayer. C3 exoenzyme (a Rho inhibitor) inhibited EC migration under static and flow conditions, and markedly reduced the effects of flow on EC migration. These results indicate that flow promotes EC migration through the Rho signaling pathway. Genistein (a tyrosine kinase inhibitor) selectively retarded EC migration under disturbed flow, suggesting that tyrosine phosphorylation may play a role in EC migration under disturbed flow. This study has demonstrated that different flow patterns differentially affect EC monolayer migration into the denuded zone involving multiple mechanisms.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5221